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Describe the various types of vectors used in DNA cloning. Expl... | Filo
Describe the various types of vectors used in DNA cloning. Expl…
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SOLVED:When cloning a foreign DNA fragment into a plasmid, it is often useful to insert the fragment at a site that interrupts a selectable marker (such as the tetracycline-resistance gene of pBR322). The loss of function of the interrupted gene can be used to identify clones containing recombinant plasmids with foreign DNA. With a bacteriophage λvector it is not necessary to do this, yet one can easily distinguish vectors that incorporate large foreign DNA fragments from those that do not. How
SOLVED:When cloning a foreign DNA fragment into a plasmid, it is …
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